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SP1 Antibody

SP1 Antibody

Item no.	ABC-AW5076-U100
Manufacturer	Abcepta
Amount	100 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applications	WB, FC, IF
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SP1, TSFP1, Transcription factor Sp1
Available
Primary Accession	P08047
Antigen Type	Recombinant Protein
Application	IF, FC, WB
Bio Background	Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression.
Bio References	Haggart M.H., et al.Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases. Takahara T., et al.J. Biol. Chem. 275:38067-38072(2000). Kadonaga J.T., et al.Cell 51:1079-1090(1987). Nicolas M., et al.Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases. Handschug K., et al.Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
Clonality	Monoclonal
Gene ID	6667
Gene Name	SP1
Subtitle	Purified Mouse Monoclonal Antibody (Mab)
Reactivity	H
Legend image 1	Fluorescent image of Hela cells stained with SP1 Antibody(Cat#AW5076). AW5076 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Type image 1	IF
Dilution image 1	1:25
Legend image 2	Western blot analysis of lysates from Hela, Ramos cell line (from left to right), using SP1 Antibody(Cat. #AW5076). AW5076 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Flow cytometric analysis of Hela cells using SP1 Antibody(green, Cat#AW5076) compared to an isotype control of mouse IgG1(blue). AW5076 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Type image 3	FC
Dilution image 3	1:25
Isotype	IgG1, κ
Calculated MW	80693
Antigen Source	HUMAN
Target/Specificity	This SP1 antibody is generated from a mouse immunized with a recombination protein from the human SP1.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ul

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Delivery expected until 9/6/2024