Item no. |
ABC-ATB10034-U100 |
Manufacturer |
Abcepta
|
Amount |
100ug |
Category |
|
Type |
Antibody Primary |
Format |
APC |
Applications |
FC |
Clone |
AFS98 |
Specific against |
other |
Host |
Rat |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
CSF1R, M-CSF Receptor, FMS, CD115, CSF-1-R, Colony-Stimulating Factor 1 Receptor |
Available |
|
Primary Accession |
P09581 |
Application |
FC |
Bio Background |
The AFS98 antibody is specific for mouse CD115, also known as Colony-Stimulating Factor-1 Receptor (CSF-1R), a 145 kDa receptor from the PDGF receptor family. Receptor activation by the ligands IL-34 or CSF-1 (M-CSF) occurs via homodimerization of CD115 and subsequent tyrosine phosphorylation and ubiquitination of intracellular domains. CD115 signaling promotes differentiation of myeloid precursors, as well as the continued regulation of proliferation, survival and function of mononuclear phagocytes, dendritic cells and osteoclasts. While IL-34 and CSF-1 may induce similar cellular responses, they are differentially expressed and as such exert complimentary actions via CD115. The AFS98 antibody may be used for identification of myeloid lineage cells by flow cytometry, and is commonly used for in vivo or in vitro neutralization of CSF-1 Receptor. |
Gene ID |
12978 |
Gene Name |
Csf1r |
Reactivity |
M |
Legend image 1 |
C57Bl/6 peripheral blood cells were stained with FITC Anti-Mouse CD11b (35-0112) and 0.06 ug APC Anti-Mouse CD115 (ATB10034) (right panel) or 0.06 ug APC Rat IgG2a (left panel). |
Type image 1 |
FC |
Other Accession |
P09581 |
Isotype |
Rat IgG2a, kappa |
Preparation |
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |
Formulation |
10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2 |
Description |
The AFS98 antibody is specific for mouse CD115, also known as Colony-Stimulating Factor-1 Receptor (CSF-1R), a 145 kDa receptor from the PDGF receptor family. Receptor activation by the ligands IL-34 or CSF-1 (M-CSF) occurs via homodimerization of CD115 and subsequent tyrosine phosphorylation and ubiquitination of intracellular domains. CD115 signaling promotes differentiation of myeloid precursors, as well as the continued regulation of proliferation, survival and function of mononuclear phagocytes, dendritic cells and osteoclasts. While IL-34 and CSF-1 may induce similar cellular responses, they are differentially expressed and as such exert complimentary actions via CD115.The AFS98 antibody may be used for identification of myeloid lineage cells by flow cytometry, and is commonly used for in vivo or in vitro neutralization of CSF-1 Receptor (use format appropriate for functional assays). |
|
Clone: AFS98 |
Application Note |
This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. |
Alternative Name(s) |
CSF-1-R, FMS, Colony-Stimulating Factor 1 Receptor, M-CSF Receptor, CSF1R |
Storage Conditions |
2-8°C protected from light |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.