Comparison

DAF Antibody (monoclonal) (M01)

Item no. ABC-AT1709a
Manufacturer Abcepta
Amount 100 ug
Category
Type Antibody Primary
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
Applications WB, ELISA
Clone 1G3
Specific against other
Host Mouse
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products CD55, CR, DAF, Complement decay-accelerating factor
Available
Primary Accession
P08174
Antigen Type
Recombinant Protein
Application
WB, E
Bio Background
This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Bio References
1.A novel form of Total Internal Reflection Fluorescence Microscopy (LG-TIRFM) reveals different and independent lipid raft domains in living cells.Asanov A, Zepeda A, Vaca L.Biochim Biophys Acta. 2009 Oct 17. [Epub ahead of print]
Clonality
Monoclonal
Gene ID
1604
Gene Name
CD55
Subtitle
Mouse monoclonal antibody raised against a partial recombinant DAF.
Reactivity
H
Legend image 1
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
Type image 1
WB
Dilution image 1
1:500~1000
Legend image 2
DAF monoclonal antibody (M01), clone 1G3 Western Blot analysis of DAF expression in HeLa ( (Cat # AT1709a )
Type image 2
WB
Dilution image 2
1:500~1000
Legend image 3
Western Blot analysis of CD55 expression in transfected 293T cell line by DAF monoclonal antibody (M01), clone 1G3.

Lane 1: CD55 transfected lysate(41.4 KDa).
Lane 2: Non-transfected lysate.
Type image 3
WB
Dilution image 3
1:500~1000
Legend image 4
Detection limit for recombinant GST tagged DAF is approximately 0.03ng/ml as a capture antibody.
Type image 4
E
Legend image 5
Proximity Ligation Analysis of protein-protein interactions between LCK and CD55. HeLa cells were stained with anti-LCK rabbit purified polyclonal 1:1200 and anti-CD55 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Other Accession
NM_000574
Isotype
IgG1 Kappa
Antigen Source
Human: ~~ Positive
Target/Specificity
DAF (NP_000565, 35 a.a. ~ 134 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
NCBI Accession
NP_000565.1; NP_001108224.1
Clone: 1G3
Org Accession
NP_000565
Gene Description
CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Gene Summary
This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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