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CPSF3 Antibody (monoclonal) (M01)

CPSF3 Antibody (monoclonal) (M01)

Item no.	ABC-AT1610a
Manufacturer	Abcepta
Amount	100 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Clear, colorless solution in phosphate buffered saline, pH 7.2 .
Applications	WB, IF, ELISA
Clone	6000000
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	CPSF3, CPSF73, Cleavage and polyadenylation specificity factor subunit 3, Cleavage and polyadenylation specificity factor 73 kDa subunit, mRNA 3'-end-processing endonuclease CPSF-73, 3127-, CPSF 73 kDa subunit
Available
Primary Accession	Q9UKF6
Antigen Type	Recombinant Protein
Application	WB, IF, E
Bio Background	Most eukaryotic mRNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3-prime end. CPSF3 encodes the 73-kD subunit of the cleavage and polyadenylation specificity factor (CPSF) and is the pre-mRNA 3-prime-end-processing endonuclease (Mandel et al., 2006 [PubMed 17128255]).
Bio References	The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3' mRNA processing factors. Rozenblatt-Rosen O, et al. Proc Natl Acad Sci U S A, 2009 Jan 20. PMID 19136632.Studies of the 5' exonuclease and endonuclease activities of CPSF-73 in histone pre-mRNA processing. Yang XC, et al. Mol Cell Biol, 2009 Jan. PMID 18955505.CSR1 induces cell death through inactivation of CPSF3. Zhu ZH, et al. Oncogene, 2009 Jan 8. PMID 18806823.Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3'-end maturation. Kolev NG, et al. EMBO Rep, 2008 Oct. PMID 18688255.The 73 kDa subunit of the CPSF complex binds to the HIV-1 LTR promoter and functions as a negative regulatory factor that is inhibited by the HIV-1 Tat protein. de la Vega L, et al. J Mol Biol, 2007 Sep 14. PMID 17669424.
Clonality	Monoclonal
Gene ID	51692
Gene Name	CPSF3
Subtitle	Mouse monoclonal antibody raised against a partial recombinant CPSF3.
Reactivity	H, M, Rat
Legend image 1	Immunofluorescence of monoclonal antibody to CPSF3 on HeLa cell. [antibody concentration 10 ug/ml]
Type image 1	IF
Legend image 2	Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
Type image 2	WB
Dilution image 2	1:500~1000
Legend image 3	CPSF3 monoclonal antibody (M01), clone 6E6. Western Blot analysis of CPSF3 expression in PC-12((Cat # AT1610a )
Type image 3	WB
Dilution image 3	1:500~1000
Legend image 4	CPSF3 monoclonal antibody (M01), clone 6E6 Western Blot analysis of CPSF3 expression in Hela S3 NE ( (Cat # AT1610a )
Type image 4	WB
Dilution image 4	1:500~1000
Legend image 5	CPSF3 monoclonal antibody (M01), clone 6E6. Western Blot analysis of CPSF3 expression in Raw 264.7((Cat # AT1610a )
Type image 5	WB
Dilution image 5	1:500~1000
Legend image 6	CPSF3 monoclonal antibody (M01), clone 6E6. Western Blot analysis of CPSF3 expression in NIH/3T3((Cat # AT1610a )
Type image 6	WB
Dilution image 6	1:500~1000
Legend image 7	Western Blot analysis of CPSF3 expression in transfected 293T cell line by CPSF3 monoclonal antibody (M01), clone 6E6. Lane 1: CPSF3 transfected lysate(77.5 KDa). Lane 2: Non-transfected lysate.
Type image 7	WB
Dilution image 7	1:500~1000
Other Accession	NM_016207
Isotype	IgG2a Kappa
Antigen Source	Human: ~~ Positive
Target/Specificity	CPSF3 (NP_057291, 585 a.a. ~ 684 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
NCBI Accession	NP_057291.1
	Clone: 6000000
Legend image 8	Detection limit for recombinant GST tagged CPSF3 is approximately 0.1ng/ml as a capture antibody.
Org Accession	NP_057291
Gene Description	cleavage and polyadenylation specific factor 3, 73kDa

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 11/8/2024

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