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CBX5 Antibody (monoclonal) (M01)

CBX5 Antibody (monoclonal) (M01)

Item no.	ABC-AT1411a
Manufacturer	Abcepta
Amount	100 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Clear, colorless solution in phosphate buffered saline, pH 7.2 .
Applications	WB, IF, ELISA
Clone	1E11-3A10
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	CBX5, HP1A, Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha
Available
Primary Accession	P45973
Antigen Type	Recombinant Protein
Application	WB, IF, E
Bio Background	This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Bio References	Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation. Nozawa RS, et al. Nat Cell Biol, 2010 Jul. PMID 20562864.Lamin A rod domain mutants target heterochromatin protein 1alpha and beta for proteasomal degradation by activation of F-box protein, FBXW10. Chaturvedi P, et al. PLoS One, 2010 May 13. PMID 20498703.Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes. Kiyomitsu T, et al. J Cell Biol, 2010 Mar 22. PMID 20231385.Protein complex of Drosophila ATRX/XNP and HP1a is required for the formation of pericentric beta-heterochromatin in vivo. Emelyanov AV, et al. J Biol Chem, 2010 May 14. PMID 20154359.ATRX interacts with H3.3 in maintaining telomere structural integrity in pluripotent embryonic stem cells. Wong LH, et al. Genome Res, 2010 Mar. PMID 20110566.
Clonality	Monoclonal
Gene ID	23468
Gene Name	CBX5
Subtitle	Mouse monoclonal antibody raised against a full length recombinant CBX5.
Reactivity	H
Legend image 1	Immunofluorescence of monoclonal antibody to CBX5 on HeLa cell. [antibody concentration 10 ug/ml]
Type image 1	IF
Legend image 2	Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (46.75 KDa) .
Type image 2	WB
Dilution image 2	1:500~1000
Legend image 3	CBX5 monoclonal antibody (M01), clone 1E11-3A10 Western Blot analysis of CBX5 expression in Hela S3 NE ( (Cat # AT1411a )
Type image 3	WB
Dilution image 3	1:500~1000
Legend image 4	Western Blot analysis of CBX5 expression in transfected 293T cell line by CBX5 monoclonal antibody (M01), clone 1E11-3A10. Lane 1: CBX5 transfected lysate(22.2 KDa). Lane 2: Non-transfected lysate.
Type image 4	WB
Dilution image 4	1:500~1000
Legend image 5	Detection limit for recombinant GST tagged CBX5 is approximately 0.03ng/ml as a capture antibody.
Type image 5	E
Other Accession	BC006821
Isotype	IgG1 kappa
Antigen Source	Human: ~~ Positive
Target/Specificity	CBX5 (AAH06821, 1 a.a. ~ 191 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
NCBI Accession	NP_001120793.1; NP_001120794.1; NP_036249.1
	Clone: 1E11-3A10
Org Accession	AAH06821
Gene Description	chromobox homolog 5 (HP1 alpha homolog, Drosophila)
Gene Summary	This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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