Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Secondary |
Specific against |
other |
Amount |
20 mg |
Host |
Rabbit |
Item no. |
ABC-ASR3456 |
Conjugate/Tag |
HRP |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Clonality |
Polyclonal |
Subtitle |
Rabbit Polyclonal, Peroxidase (Horseradish) |
Immunogen |
Hamster IgG whole molecule |
Concentration (mg/ml) |
10.0 mg/mL by UV absorbance at 280 nm |
Conjugate |
Peroxidase (Horseradish) |
Application Note |
ELISA 1:10, 000-1:50, 000; Western Blot 1:1, 000-1:10, 000; Immunohistochemistry 1:500-1:2, 500 |
Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Target Species |
Golden Syrian Hamster |
Physical State |
Lyophilized |
Target Isotype |
IgG (H&L) |
Host Isotype |
IgG F(ab')2 |
Reconstitution Volume |
2.0 mL |
Reconstitution Buffer |
Restore with deionized water (or equivalent) |
Stabilizer |
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative |
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Shipping Condition |
Ambient |
Purity |
This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Hamster IgG and Hamster Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin. |
Storage Condition |
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.