Item no. |
ABC-ASR2973 |
Manufacturer |
Abcepta
|
Amount |
1 mg |
Category |
|
Type |
Antibody Secondary |
Specific against |
other |
Host |
Donkey |
Conjugate/Tag |
HRP |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Available |
|
Bio Background |
Anti-Sheep IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically sheep IgG. This secondary peroxidase conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. |
Clonality |
Polyclonal |
Subtitle |
Donkey Polyclonal, Peroxidase (Horseradish) |
Reactivity |
Sh |
Immunogen |
Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey. |
Concentration (mg/ml) |
1.0 mg/mL by UV absorbance at 280 nm |
Conjugate |
Peroxidase (Horseradish) |
Application Note |
ELISA 1:10, 000-1:50, 000; Western Blot 1:1, 000-1:10, 000; Immunochemistry 1:500-1:2, 500 |
Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Target Species |
Sheep |
Physical State |
Lyophilized |
Target Isotype |
IgG (H&L) |
Host Isotype |
IgG |
Reconstitution Volume |
1.0 mL |
Reconstitution Buffer |
Restore with deionized water (or equivalent) |
Stabilizer |
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative |
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Shipping Condition |
Ambient |
Purity |
Anti-Sheep IgG (H&L) peroxidase conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against Chicken, Guinea Pig, Golden Syrian Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins. |
Storage Condition |
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.