Comparison

Anti-Sheep IgG (H&L) (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody

Item no. ABC-ASR2973
Manufacturer Abcepta
Amount 1 mg
Category
Type Antibody Secondary
Specific against other
Host Donkey
Conjugate/Tag HRP
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
Bio Background
Anti-Sheep IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically sheep IgG. This secondary peroxidase conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Clonality
Polyclonal
Subtitle
Donkey Polyclonal, Peroxidase (Horseradish)
Reactivity
Sh
Immunogen
Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Concentration (mg/ml)
1.0 mg/mL by UV absorbance at 280 nm
Conjugate
Peroxidase (Horseradish)
Application Note
ELISA 1:10, 000-1:50, 000; Western Blot 1:1, 000-1:10, 000; Immunochemistry 1:500-1:2, 500
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Target Species
Sheep
Physical State
Lyophilized
Target Isotype
IgG (H&L)
Host Isotype
IgG
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Shipping Condition
Ambient
Purity
Anti-Sheep IgG (H&L) peroxidase conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Sheep IgG and Sheep Serum.  No reaction was observed against Chicken, Guinea Pig, Golden Syrian Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 mg
Available: In stock
available

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