Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Secondary |
Specific against |
other |
Amount |
1 mg |
Host |
Goat |
Item no. |
ABC-ASR1960 |
Conjugate/Tag |
FITC |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Bio Background |
FITC Anti-Hamster IgG Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
Clonality |
Polyclonal |
Subtitle |
Goat Polyclonal, Fluorescein (FITC) |
Reactivity |
Ha |
Legend image 1 |
Western Blot of Fluorescein conjugated Goat anti-Armenian Hamster IgG secondary antibody.Lane 1: Armenian Hamster IgG. Lane 2: none. Load: 50 ng per lane.Primary antibody: none.Secondary antibody: Fluorescein goat secondary antibody at 1:1, 000 for 60 min at RT.Block: MB-070 for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Armenian Hamster IgG. Other band(s): none. |
Type image 1 |
WB |
Immunogen |
Armenian Hamster IgG whole molecule |
Concentration (mg/ml) |
1.0 mg/mL by UV absorbance at 280 nm |
Conjugate |
Fluorescein (FITC) |
Application Note |
FLISA 1:10, 000-1:50, 000; IF Microscopy 1:1, 000-1:5, 000; FlowCytometry 1:500-1:2, 500; |
Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
FP Value |
2.8 moles Fluorescein (FITC) per mole of IgG |
Target Species |
Armenian Hamster |
Physical State |
Lyophilized |
Target Isotype |
IgG (H&L) |
Host Isotype |
IgG |
Reconstitution Volume |
1.0 mL |
Reconstitution Buffer |
Restore with deionized water (or equivalent) |
Stabilizer |
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative |
0.01% (w/v) Sodium Azide |
Shipping Condition |
Ambient |
Purity |
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum. Minimal reactivity will occur against Golden Syrian Hamster IgG. |
Storage Condition |
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.