Comparison

F(ab')2 Anti-Cat IgG (H&L) Secondary Antibody

Manufacturer Abcepta
Category
Type Antibody Secondary
Specific against other
Amount 1 mg
Host Rabbit
Item no. ABC-ASR1843
eClass 6.1 32160702
eClass 9.0 32160702
Available
Bio Background
F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Clonality
Polyclonal
Subtitle
Rabbit Polyclonal, Unconjugated
Immunogen
Cat IgG whole molecule
Conjugate
Unconjugated
Application Note
ELISA 1:20, 000-1:100, 000; Western Blot 1:2, 000-1:10, 000; Immunohistochemistry 1:1, 000-1:5, 000
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Target Species
Cat
Physical State
Liquid (sterile filtered)
Target Isotype
IgG (H&L)
Host Isotype
IgG F(ab')2
Stabilizer
None
Shipping Condition
Wet Ice
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Cat IgG and Cat Serum.  No reaction was observed against anti-Pepsin and anti-Rabbit IgG F(c).
Storage Condition
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 mg
Available: In stock
available

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