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Anti-Rabbit IgG F(ab')2 (Rhodamine Conjugated) Secondary Antibody

Anti-Rabbit IgG F(ab')2 (Rhodamine Conjugated) Secondary Antibody

Item no.	ABC-ASR1813
Manufacturer	Abcepta
Amount	2 mg
Category	Antibodies Secondary Antibodies
Type	Antibody Secondary
Specific against	other
Host	Goat
Conjugate/Tag	Rhodamine
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Bio Background	This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Clonality	Polyclonal
Subtitle	Goat Polyclonal, Rhodamine (TRITC)
Legend image 1	Western Blot of Anti-Rabbit IgG F(ab')2 (GOAT) Antibody . Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule . Lane 2: Rabbit IgG F(ab) Fragment . Lane 3: Rabbit IgG F(c) Fragment . Lane 4: Rabbit IgM Whole Molecule . Lane 5: Normal Rabbit Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(ab')2 (GOAT) Antibody 1:10, 000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40, 000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Type image 1	WB
Immunogen	Rabbit IgG F(ab' )2 fragment
Concentration (mg/ml)	2.0 mg/mL by UV absorbance at 280 nm
Conjugate	Rhodamine (TRITC)
Application Note	FLISA 1:10, 000-1:50, 000; IF Microscopy 1:1, 000-1:5, 000; FlowCytometry 1:500-1:2, 500
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
FP Value	2.5 moles Rhodamine (TRITC) per mole of IgG
Target Species	Rabbit
Physical State	Lyophilized
Target Isotype	IgG F(ab')2
Host Isotype	IgG
Reconstitution Volume	1.0 mL
Reconstitution Buffer	Restore with deionized water (or equivalent)
Stabilizer	10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative	0.01% (w/v) Sodium Azide
Shipping Condition	Ambient
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c).
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 2 mg

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Delivery expected until 10/4/2024

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