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Anti-Mouse IgG F(c) (Fluorescein Conjugated) Secondary Antibody

Anti-Mouse IgG F(c) (Fluorescein Conjugated) Secondary Antibody

Manufacturer	Abcepta
Category	Antibodies Secondary Antibodies
Type	Antibody Secondary
Specific against	other
Amount	2 mg
Host	Goat
Item no.	ABC-ASR1799
Conjugate/Tag	FITC
eClass 6.1	32160702
eClass 9.0	32160702
Available
Bio Background	This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Clonality	Polyclonal
Subtitle	Goat Polyclonal, Fluorescein (FITC)
Legend image 1	Western blot of Fluorescein conjugated Goat Anti-Mouse IgG F(c) secondary antibody. Lane 1: Mouse IgG F(c). Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1, 000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Mouse IgG F(c). Other band(s): None.
Type image 1	WB
Immunogen	Mouse IgG F(c) fragment
Concentration (mg/ml)	2.0 mg/mL by UV absorbance at 280 nm
Conjugate	Fluorescein (FITC)
Application Note	FLISA 1:10, 000-1:50, 000; IF Microscopy 1:1, 000-1:5, 000; FlowCytometry 1:500-1:2, 500
Buffer	0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
FP Value	3.1 moles Fluorescein (FITC) per mole of IgG
Target Species	Mouse
Physical State	Lyophilized
Target Isotype	IgG F(c)
Host Isotype	IgG
Reconstitution Volume	1.0 mL
Reconstitution Buffer	Restore with deionized water (or equivalent)
Stabilizer	10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative	0.01% (w/v) Thimerosal
Shipping Condition	Ambient
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab’)2.
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 2 mg

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Delivery expected until 9/6/2024

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