Comparison

Chicken IgG Fab Rhodamine

Item no. ABC-ASR1737
Manufacturer Abcepta
Amount 1 mg
Category
Type Antibody Primary
Specific against other
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
Bio Background
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Legend image 1
SDS-PAGE of Chicken IgG F(ab) Fragment Rhodamine Conjugated . Lane M: 5 µL Opal Prestained Marker . Lane 1: Reduced Chicken IgG Whole Molecule . Lane 2: Reduced Chicken IgG F(c) Fragment . Lane 3: Reduced Chicken IgG F(ab) Fragment Rhodamine Conjugated . Lane 4: Reduced Chicken IgM Whole Molecule . Load: 1 µg per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.
Conjugate
Rhodamine (TRITC)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State
Lyophilized
Host Isotype
IgG F(ab)
Species of Origin
Chicken
PSC Type
Protein
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% (w/v) Sodium Azide
Shipping Condition
Ambient
Purity
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG, anti-Chicken IgG F(ab’)2 and anti-Chicken Serum.  No reaction was observed against anti-Chicken IgG F(c) or anti-Pepsin.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 mg
Available: In stock
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