Item no. |
ABC-ASR1671 |
Manufacturer |
Abcepta
|
Amount |
2 mg |
Category |
|
Type |
Antibody Secondary |
Specific against |
other |
Host |
Donkey |
Conjugate/Tag |
Texas Red |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Available |
|
Bio Background |
Sheep IgG (H&L) Antibody Texas Red™ Conjugated antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
Clonality |
Polyclonal |
Subtitle |
Donkey Polyclonal, Texas Red® |
Legend image 1 |
Western Blot of Donkey anti-Sheep IgG Texas Red Conjugated Antibody. Lane 1: Sheep IgG. Lane 2: None. Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Texas Red donkey secondary antibody at 1:1, 000 for 60 min at RT.Block: MB-070 for 30 min at RT.Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Sheep IgG. Other band(s): None. |
Type image 1 |
WB |
Immunogen |
Sheep IgG whole molecule |
Concentration (mg/ml) |
2.0 mg/mL by UV absorbance at 280 nm |
Conjugate |
Texas Red® |
Application Note |
FLISA 1:10, 000-1:50, 000; IF Microscopy 1:1, 000-1:5, 000; FlowCytometry 1:500-1:2, 500 |
Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
FP Value |
4.7 moles Texas Red® per mole of IgG |
Target Species |
Sheep |
Physical State |
Lyophilized |
Target Isotype |
IgG (H&L) |
Host Isotype |
IgG |
Reconstitution Volume |
1.0 mL |
Reconstitution Buffer |
Restore with deionized water (or equivalent) |
Stabilizer |
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative |
0.01% (w/v) Sodium Azide |
Shipping Condition |
Ambient |
Purity |
Sheep IgG (H&L) Antibody Texas Red™ Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum. |
Storage Condition |
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.