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Anti-Human IgG (H&L) (Biotin Conjugated) Secondary Antibody

Anti-Human IgG (H&L) (Biotin Conjugated) Secondary Antibody

Manufacturer	Abcepta
Category	Antibodies Secondary Antibodies
Type	Antibody Secondary
Specific against	other
Amount	2 mg
Host	Rabbit
Item no.	ABC-ASR1606
Conjugate/Tag	Biotin
eClass 6.1	32160702
eClass 9.0	32160702
Available
Clonality	Polyclonal
Subtitle	Rabbit Polyclonal, Biotin
Legend image 1	Western Blot of Rabbit anti-Human IgG (H&L) Antibody Biotin Conjugated Lane 1: Human IgG Load: 50 ng per lane Primary antibody: Human IgG (H&L) Antibody Biotin Conjugated at 1:1000 for 60 min RT Secondary antibody: HRP Conjugated Streptavidin at 1:40, 000 for 30 min at RT Block: MB-070 for 30 min at RT
Type image 1	WB
Dilution image 1	1:2, 000 - 1:10, 000
Immunogen	Human IgG whole molecule
Concentration (mg/ml)	2.0 mg/mL by UV absorbance at 280 nm
Conjugate	Biotin
Application Note	ELISA 1:20, 000-1:100, 000; Western Blot 1:2, 000-1:10, 000; Immunochemistry 1:1, 000-1:5, 000
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Target Species	Human
Physical State	Lyophilized
Target Isotype	IgG (H&L)
Host Isotype	IgG
Reconstitution Volume	1.0 mL
Reconstitution Buffer	Restore with deionized water (or equivalent)
Stabilizer	10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative	0.01% (w/v) Sodium Azide
Shipping Condition	Ambient
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgG and Human Serum.
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASR1606-datasheet.pdf

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Amount: 2 mg

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Delivery expected until 9/6/2024

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