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BDNF Antibody

BDNF Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	ATTO 594
Amount	Inquiry
Item no.	ABC-ASM10558-A594-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	P23560
Reactivity	H
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BDNF Polyclonal Antibody (ASM10558). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (ASM10558) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Secreted, Cytoplasm, Membrane-bound vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BDNF Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western blot analysis of Human HeLa and 293T cell lysates showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody (ASM10558). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (ASM10558) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of Mouse Brain showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody (ASM10558). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (ASM10558) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.
Type image 3	WB
Dilution image 3	1:1000
Other Accession	NP_001137277.1
Target/Specificity	Predicted molecular weight at ~27.9kDa.
Immunogen	Synthetic peptide from the N-terminal of human BDNF
Purification	Peptide Affinity Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10558-A594-100-datasheet.pdf

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