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BNIP3 Antibody

BNIP3 Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	RPE
Amount	Inquiry
Item no.	ABC-ASM10545-RPE-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q12983
Reactivity	H
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BNIP3 Polyclonal Antibody (ASM10545). Tissue: Fibroblast cell line (NIH 3T3). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (ASM10545) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BNIP3 Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western blot analysis of Human HeLa and 293T cell lysates showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (ASM10545). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (ASM10545) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa .
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of Mouse Kidney showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (ASM10545). Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (ASM10545) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa . Other Band(s): ~60kDa (BNIP3 complex).
Type image 3	WB
Dilution image 3	1:1000
Other Accession	NP_004043.3
Target/Specificity	Predicted molecular weight at ~21.5kDa. Observed bands at ~20-30kDa and 50-60kDa due to the homodimer complex of BNIP3.
Immunogen	Synthetic peptide from the C-terminal of human BNIP3
Purification	Peptide Affinity Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10545-RPE-100-datasheet.pdf

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