« Back
Home /
AMBRA1 Antibody

AMBRA1 Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	PerCP
Amount	Inquiry
Item no.	ABC-ASM10523-PCP-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q9C0C7
Reactivity	H, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AMBRA1 Polyclonal Antibody (ASM10523). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody (ASM10523) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) AMBRA1 Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western blot analysis of Rat Brain cell lysates showing detection of 142.5 kDa AMBRA1 protein using Rabbit Anti-AMBRA1 Polyclonal Antibody (ASM10523). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody (ASM10523) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 142.5 kDa. Other Band(s): 50 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_001254711.1
Target/Specificity	Detects ~142 kDa.
Immunogen	Synthetic peptide from the C-terminal of Human AMBRA1 (aa. 202-212)
Purification	Peptide Affinity Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ABC-ASM10523-PCP-100-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: Inquiry

available

Delivery expected until 9/6/2024

Express Delivery: Save 30 days

Compare

Add to wishlist

Recommend by mail