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ULK1 Antibody

ULK1 Antibody

Item no.	ABC-ASM10520-A655-100
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	ATTO 655
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	O75385
Reactivity	H, M, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ULK1 Polyclonal Antibody (ASM10520). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (ASM10520) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ULK1 Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 112.6 kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (ASM10520). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (ASM10520) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 112.6 kDa.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of Rat Brain cell lysates showing detection of ~112.6kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody (ASM10520). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Block: 2% GE Healthcare Blocker (RT, 60 minutes). Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody (ASM10520) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~112.6kDa.
Type image 3	WB
Dilution image 3	1:1000
Other Accession	NP_003556.1
Target/Specificity	Detects ~100 kDa.
Immunogen	Synthetic peptide from the mid-protein of Human ULK1 (aa. 567-577)
Purification	Peptide Affinity Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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