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ATG4D Antibody

ATG4D Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	ATTO 655
Amount	Inquiry
Item no.	ABC-ASM10515-A655-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q86TL0
Reactivity	H
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4D Polyclonal Antibody (ASM10515). Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody (ASM10515) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4D Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western blot analysis of Human HeLa cell lysates showing detection of ~52.9kDa ATG4D protein using Rabbit Anti-ATG4D Polyclonal Antibody (ASM10515). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody (ASM10515) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.9kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_001268433.1
Target/Specificity	Detects ~52kDa.
Immunogen	Synthetic peptide from the C-terminal of human ATG4D
Purification	Peptide Affinity Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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