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ATG4C Antibody

ATG4C Antibody

Item no.	ABC-ASM10514-A390-100
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	ATTO 390
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	Q96DT6
Reactivity	H
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody (ASM10514). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody (ASM10514) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG4C Antibody. (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western blot analysis of Human HEK293T cell lysates showing detection of 53 kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody (ASM10514). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HEK293T cell lysates. Load: 15 µg . Block: 2% GE Ashermsham in 1X TBST. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody (ASM10514) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 minutes in RT. Predicted/Observed Size: 53 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_116241.2
Target/Specificity	Detects ~50 kDa.
Immunogen	Synthetic peptide from the C-terminal of Human ATG4C (aa. 296-306)
Purification	Peptide Affinity Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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