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Beclin 2 Antibody

Beclin 2 Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	ATTO 594
Amount	Inquiry
Item no.	ABC-ASM10493-A594-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	P0DM65
Reactivity	H, M
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10493). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10493) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 2 Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 48.1 kDa Beclin 2 protein using Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10493). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10493) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 48.1 kDa.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of Mouse Brain cell lysates showing detection of 48.1 kDa Beclin 2 protein using Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10493). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10493) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 48.1 kDa.
Type image 3	WB
Dilution image 3	1:1000
Other Accession	NP_001277621.1
Target/Specificity	Detects ~48 kDa.
Immunogen	Synthetic peptide from the C-terminal of Mouse Beclin 2 (aa. 410-421)
Purification	Peptide Affinity Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10493-A594-100-datasheet.pdf

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