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HSF1 Antibody

HSF1 Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	Unconjugated
Amount	Inquiry
Item no.	ABC-ASM10468-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q00613
Reactivity	H, M, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSF1 Polyclonal Antibody (ASM10468). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HSF1 Polyclonal Antibody (ASM10468) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSF1 Polyclonal Antibody (ASM10468). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HSF1 Polyclonal Antibody (ASM10468) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Type image 2	ICC/IF
Dilution image 2	1:100
Other Accession	NP_005517.1
Target/Specificity	Detects ~85kDa (unstressed cell lysates), and ~95kDa (heat shocked cell lysates).
Immunogen	Purified human HSF1 protein NM_005526.2
Purification	Protein A Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10468-100-datasheet.pdf

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