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RAS Antibody

RAS Antibody

Item no.	ABC-ASM10429-100
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Unconjugated
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	B2KGV5
Reactivity	H, M, Rat, B
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (ASM10429). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (ASM10429) at 1:200 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Lipid-anchor. Focal adhesion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-RAS Antibody. (C) Composite.
Type image 1	ICC/IF
Dilution image 1	1:200
Legend image 2	Immunohistochemistry analysis using Rabbit Anti-RAS Polyclonal Antibody (ASM10429). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (ASM10429) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Basal cell of the epidermis.
Type image 2	IHC
Dilution image 2	1:100
Legend image 3	Western blot analysis of Mouse Brain Cortex cell lysates showing detection of ~21 kDa RAS protein using Rabbit Anti-RAS Polyclonal Antibody (ASM10429). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Cortex cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (ASM10429) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (ASM10429). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (ASM10429) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cell-cell junction.
Type image 4	ICC/IF
Dilution image 4	1:200
Legend image 5	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (ASM10429). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (ASM10429) at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Lipid-anchor. Focal adhesion. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-RAS Antibody. (C) Composite.
Type image 5	ICC/IF
Dilution image 5	1:200
Other Accession	NP_035067.2
Target/Specificity	Detects ~21kDa.
Immunogen	Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Delivery expected until 10/4/2024

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