Comparison

TNF-R1 Antibody

Item no. ABC-ASM10426-A655-100
Manufacturer Abcepta
Amount Inquiry
Category
Type Antibody Primary
Format ATTO 655
Specific against other
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
Primary Accession
P19438
Reactivity
H, M, Rat, Rb, Pr, B, Dog
Legend image 1
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Golgi apparatus membrane. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TNF-R1 Antibody. (C) Composite.
Type image 1
ICC/IF
Dilution image 1
1:100
Legend image 2
Immunohistochemistry analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: dermis.
Type image 2
IHC
Dilution image 2
1:100
Legend image 3
Western blot analysis of Mouse Liver cell lysates showing detection of ~55 kDa TNF-R1 protein using Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55 kDa.
Type image 3
WB
Dilution image 3
1:1000
Legend image 4
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Punctate nuclear staining, dotty staining in cytoplasm.
Type image 4
ICC/IF
Dilution image 4
1:100
Legend image 5
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Golgi apparatus membrane. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TNF-R1 Antibody. (C) Composite.
Type image 5
ICC/IF
Dilution image 5
1:100
Other Accession
P19438
Target/Specificity
Detects ~55kDa.
Immunogen
Peptide corresponding to AA 20-43 of the mouse TNF-R1 sequence, identical to rat and human over those residues
Purification
Peptide Affinity Purified
Reconstitution & Storage
-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: Inquiry
Available: In stock
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