Item no. |
ABC-ASM10426-A655-100 |
Manufacturer |
Abcepta
|
Amount |
Inquiry |
Category |
|
Type |
Antibody Primary |
Format |
ATTO 655 |
Specific against |
other |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Available |
|
Primary Accession |
P19438 |
Reactivity |
H, M, Rat, Rb, Pr, B, Dog |
Legend image 1 |
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Golgi apparatus membrane. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TNF-R1 Antibody. (C) Composite. |
Type image 1 |
ICC/IF |
Dilution image 1 |
1:100 |
Legend image 2 |
Immunohistochemistry analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: dermis. |
Type image 2 |
IHC |
Dilution image 2 |
1:100 |
Legend image 3 |
Western blot analysis of Mouse Liver cell lysates showing detection of ~55 kDa TNF-R1 protein using Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55 kDa. |
Type image 3 |
WB |
Dilution image 3 |
1:1000 |
Legend image 4 |
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Punctate nuclear staining, dotty staining in cytoplasm. |
Type image 4 |
ICC/IF |
Dilution image 4 |
1:100 |
Legend image 5 |
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (ASM10426) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Golgi apparatus membrane. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TNF-R1 Antibody. (C) Composite. |
Type image 5 |
ICC/IF |
Dilution image 5 |
1:100 |
Other Accession |
P19438 |
Target/Specificity |
Detects ~55kDa. |
Immunogen |
Peptide corresponding to AA 20-43 of the mouse TNF-R1 sequence, identical to rat and human over those residues |
Purification |
Peptide Affinity Purified |
Reconstitution & Storage |
-20ºC |
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