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Rab5 Antibody

Rab5 Antibody

Item no.	ABC-ASM10422-ALP-100
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Alkaline Phosphatase
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	Q6FI44
Reactivity	H, M, Rat, Pr, B
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422) at 1:80 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody. (C) Composite.
Type image 1	ICC/IF
Dilution image 1	1:80
Legend image 2	Western blot analysis of Human Cell line lysates showing detection of Rab5 protein using Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Immunohistochemistry analysis using Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.
Type image 3	IHC
Dilution image 3	1:100
Legend image 4	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody (ASM10422) at 1:80 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Rab5 Antibody. (C) Composite.
Type image 4	ICC/IF
Dilution image 4	1:80
Other Accession	NP_004153.2
Target/Specificity	Detects ~26kDa.
Immunogen	Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues
Purification	Protein A Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10422-ALP-100-datasheet.pdf

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