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GABA A Receptor Alpha 5 Antibody

GABA A Receptor Alpha 5 Antibody

Item no.	ABC-ASM10326-PCP-100
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	PerCP
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	P31644
Reactivity	H, M, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody, Clone S415-24 (ASM10326). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody (ASM10326) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-A Receptor Alpha5 Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone S415-24 (ASM10326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 µg. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody (ASM10326) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_000801.1
Isotype	IgG1
Target/Specificity	Detects ~55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
Immunogen	Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5.
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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