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Protocadherin Gamma A3 Antibody

Protocadherin Gamma A3 Antibody

Item no.	ABC-ASM10285-PCP-100
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	PerCP
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	Q91XY5
Reactivity	H, M
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody, Clone S144-17 (ASM10285). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody (ASM10285) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma A3 Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 showing detection of ~100 kDa Protocadherin Gamma A3 protein using Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody, Clone S144-17 (ASM10285). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody (ASM10285) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_291064.1
Isotype	IgG1
Target/Specificity	Detects ~100kDa. No cross-reactivity against other Protocadherin Gamma -A, -B or -C proteins.
Immunogen	Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma- protocadherin-A3. Human: 80% identity (68/84 amino acids identical) >75% identity with other Gamma-protocadherin-A protein.
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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