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Pannexin-2 Antibody

Pannexin-2 Antibody

Item no.	ABC-ASM10254-APC-100
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	APC
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	P60571
Reactivity	H, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Pannexin-2 Monoclonal Antibody, Clone S121A-31 (ASM10254). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Pannexin-2 Monoclonal Antibody (ASM10254) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Rat Brain Membrane showing detection of ~70 kDa Pannexin 2 protein using Mouse Anti-Pannexin 2 Monoclonal Antibody, Clone S121A-31 (ASM10254). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Pannexin 2 Monoclonal Antibody (ASM10254) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~70 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_955441.2
Isotype	IgG1
Target/Specificity	Detects ~70kDa. Does not cross-react with Pannexin-1 or Pannexin-3.
Immunogen	Fusion protein amino acids 11-664 (all but first 10 amino acids, encompassing extracellular and cytoplasmic domains) of rat Pannexin-2
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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