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LRP4 (Cytoplasmic) Antibody

LRP4 (Cytoplasmic) Antibody

Item no.	ABC-ASM10249-A633-100
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	ATTO 633
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	Q8VI56
Reactivity	H, M, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP4 (Cytoplasmic) Monoclonal Antibody, Clone S164-6 (ASM10249). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRP4 (Cytoplasmic) Monoclonal Antibody (ASM10249) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) LRP4 (Cytoplasmic) Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa LRP4 protein using Mouse Anti-LRP4 Monoclonal Antibody, Clone S164-6 (ASM10249). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-LRP4 Monoclonal Antibody (ASM10249) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_766256.3
Isotype	IgG1
Target/Specificity	Detects the cytoplasmic domain of LRP4 ~215kDa, small degradation products ~100kD.
Immunogen	Fusion protein amino acids 1747-1905 (cytoplasmic C-terminus) of mouse LRP4
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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