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SHANK (pan) Antibody

SHANK (pan) Antibody

Item no.	ABC-ASM10203-A488-100
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	ATTO 488
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	Q9QX74
Reactivity	H, M, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK (pan) Monoclonal Antibody, Clone S23b-49 (ASM10203). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK (pan) Monoclonal Antibody (ASM10203) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SHANK (pan) Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Rat brain membrane lysate showing detection of SHANK protein using Mouse Anti-SHANK Monoclonal Antibody, Clone S23b-49 (ASM10203). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK Monoclonal Antibody (ASM10203) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Type image 2	WB
Dilution image 2	1:1000
Isotype	IgG1
Target/Specificity	Detects ~160kDa. Recognizes Shank1, 2 and 3.
Immunogen	Fusion protein amino acids 84-309 of rat Shank2
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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