Item no. |
ABC-ASM10178-FITC-100 |
Manufacturer |
Abcepta
|
Amount |
Inquiry |
Category |
|
Type |
Antibody Primary |
Format |
FITC |
Specific against |
other |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Available |
|
Primary Accession |
P15381 |
Reactivity |
H, M, Rat, Ha |
Legend image 1 |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57-46 (ASM10178). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (ASM10178) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-Cav1.2 Antibody. (C) Composite. |
Type image 1 |
ICC/IF |
Dilution image 1 |
1:100 |
Legend image 2 |
Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 (ASM10178). Tissue: hippocampus. Species: Human. Fixation: 10% formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (ASM10178) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. |
Type image 2 |
IHC |
Dilution image 2 |
1:1000 |
Legend image 3 |
Immunoprecipitation analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 (ASM10178). Tissue: INS-1E cells. Species: Rat. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (ASM10178) at 1:200. Courtesy of: Merrie Mosedale. |
Type image 3 |
IP |
Legend image 4 |
Western Blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 (ASM10178). Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (ASM10178) at 1:1000. |
Type image 4 |
WB |
Dilution image 4 |
1:1000 |
Legend image 5 |
Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody, Clone S57-47 (ASM10178). Tissue: Brain Tissue. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody (ASM10178) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. |
Type image 5 |
IHC |
Dilution image 5 |
1:1000 |
Other Accession |
NP_001129994.1 |
Isotype |
IgG1 |
Target/Specificity |
Detects ~240kDa (varies with cell background due to glycosylation). |
Immunogen |
Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2 |
Purification |
Protein G Purified |
Reconstitution & Storage |
-20ºC |
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