« Back
Home /
HSP70 Antibody

HSP70 Antibody

Item no.	ABC-ASM10169-HRP-100
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	HRP
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	P0DMV8
Reactivity	H, M, Rat, Pig, B
Legend image 1	Immunocytochemistry/Immunofluorescence analysis on non-permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate (ASM10169D-FITC) showing cell membrane staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: None. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (ASM10169) at 1:100. Counterstain: Wheat germ agglutinin Texas red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Fluorescence-activated cell sorting (FACS) using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate (ASM10169D-FITC). Tissue: Jurkat E6.1. Species: Human. Unfixed/non-permeabilized cells were incubated with (A) Anti-HSP70 control antibody, or (B) Anti-HSP70 antibody, clone 1H11 (ASM10169D-FITC), for 40 min at 4°C. Fixed/permeabilized cells were incubated with (C) Anti-HSP70 control antibody, or (D) Anti-HSP70 antibody, clone 1H11 (ASM10169D-FITC) for 40 min at 4°C. Analysis shows that Anti-HSP70 (ASM10169) can detect low levels of cell surface HSP70, and can faintly detect intracellular HSP70. Courtesy of: Dr. Elyse Ireland, Institute of Medicine, University of Chester.
Type image 2	FACS
Dilution image 2	1:250
Legend image 3	Western Blot analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11 (ASM10169). Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 µg. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (ASM10169) at 1:1000.
Type image 3	WB
Legend image 4	Immunocytochemistry/Immunofluorescence analysis on permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate (ASM10169D-FITC) showing faint cell membrane and intracellular staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: 0.2% PBST. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (ASM10169) at 1:100. Counterstain: Wheat germ agglutinin Texas red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, faint intracellular. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.
Type image 4	ICC/IF
Dilution image 4	1:100
Legend image 5	Fluorescence-activated cell sorting analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate (ASM10169D-FITC). Tissue: HCT116 and HK2 cells. Species: Human. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (ASM10169) at 1:100 for 90 min at 4°C. Counterstain: Propidium Iodide nuclear stain. (A) HK2 cells unstained. (B) HK2 cells and HSP70 Antibody. (C) HCT116 cells unstained. (D) HCT116 cells and HSP70 Antibody. Shows that HSP70 Antibody binds to the cell surface of tumor cells but not non-tumor cells. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.
Type image 5	FACS
Dilution image 5	1:250
Legend image 6	Fluorescence-activated cell sorting analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate (ASM10169D-FITC). Tissue: Jurkat E6.1, U937, MCF7 and HT29 cells . Species: Human. Fixation: No fixation. Permeabilization: None. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (ASM10169) at 20 µg/ml for 40 min at 4°C. Counterstain: Propidium Iodide nuclear stain at 2.5 µg/ml for 5 min at 4°C. Shows that binding of HSP70 Antibody is not cell-line specific. Courtesy of: Dr. Elyse Ireland, Institute of Medicine, University of Chester.
Type image 6	FACS
Dilution image 6	1:250
Other Accession	NP_005336.3
Isotype	IgG1
Target/Specificity	Binds to cell surface HSP70 without permeabilization of cell membrane in tumor cell lines. Does not bind to intracellular HSP70 at low concentrations. Detects a ~70 kDa band in Western Blot.
Immunogen	Human native HSP70 protein
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ABC-ASM10169-HRP-100-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: Inquiry

available

Delivery expected until 10/4/2024

Express Delivery: Save 22 days

Compare

Add to wishlist

Recommend by mail