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PP5 Antibody

PP5 Antibody

Item no.	ABC-ASM10167-100
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Unconjugated
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	P53041
Reactivity	H
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 2E11 (ASM10167). Tissue: HEK293. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody (ASM10167) at 1:50 for 1 hour at RT. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:100 for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain. Magnification: 63x.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Human A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using Mouse Anti-PP5 Monoclonal Antibody, Clone 2E11 (ASM10167). Lane 1: MW Ladder. Lane 2: Human A431 (15 µg). Lane 3: Human HEK293 (15 µg). Lane 4: Human Jurkat (15 µg). Load: 15 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody (ASM10167) at 1:500 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~58 kDa.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 2E11 (ASM10167). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody (ASM10167) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 40X.
Type image 3	ICC/IF
Dilution image 3	1:100
Other Accession	NP_006238.1
Isotype	IgG2a
Target/Specificity	Detects ~58kDa.
Immunogen	Full length human PP5 protein
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10167-100-datasheet.pdf

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