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Synaptophysin (native) Antibody

Synaptophysin (native) Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	Biotin
Amount	Inquiry
Item no.	ABC-ASM10118-BI-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	P08247
Reactivity	H
Legend image 1	Immunohistochemistry analysis using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP10 (ASM10118). Tissue: Brain Slice. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (ASM10118) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons and fibers but not on the white matter. Magnification: 20x.
Type image 1	IHC
Dilution image 1	1:1000
Legend image 2	Western Blot analysis of Human Brain showing detection of ~38 kDa Synaptophysin protein using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP11 (ASM10118). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (ASM10118) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~38 kDa. Other Band(s): 25 kDa, 20 kDa.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Immunohistochemistry analysis using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP10 (ASM10118). Tissue: Cerebellum. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (ASM10118) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons and fibers. Magnification: 20x.
Type image 3	IHC
Legend image 4	Immunohistochemistry analysis using Mouse Anti-Synaptophysin Monoclonal Antibody, Clone EP10 (ASM10118). Tissue: Fetal brain tissue. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Synaptophysin Monoclonal Antibody (ASM10118) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons.
Type image 4	IHC
Dilution image 4	1:1000
Other Accession	NP_003170.1
Isotype	IgG1
Target/Specificity	Detects ~38kDa.
Immunogen	Immunoprecipitate of human brain
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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