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Ubiquitin Antibody

Ubiquitin Antibody

Item no.	ABC-ASM10087-A565-200
Manufacturer	Abcepta
Amount	Inquiry
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	ATTO 565
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	P0CG53
Reactivity	H, M, Rat, B
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 5B9-B3 (ASM10087). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (ASM10087) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 5B9-B3 (ASM10087). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (ASM10087) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite.
Type image 2	ICC/IF
Dilution image 2	1:100
Legend image 3	Western Blot analysis of Human cell lysates showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 5B9-B3 (ASM10087). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (ASM10087) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Type image 3	WB
Other Accession	NP_776558.1
Isotype	IgG2a Kappa
Target/Specificity	Detects ~10kDa.
Immunogen	Native bovine ubiquitin, conjugated to KLH
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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