Item no. |
ABC-ASC11387 |
Manufacturer |
Abcepta
|
Amount |
100 ug |
Category |
|
Type |
Antibody Primary |
Applications |
WB, ICC, ELISA |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Available |
|
Primary Accession |
Q9H1Z8 |
Application |
WB, ICC, E |
Bio Background |
ECRG4 Antibody: The esophageal cancer-related gene 4 (ECRG4), also known as Augurin, was identified through differential display screening between esophageal cancer and normal tissue. Hypermethylation of CpG sites in the ECRG4 promoter correlate with ECRG4 downregulation in numerous esophageal squamous cell carcinoma tissues.. In transfected cells, overexpression of ECRG4 induced G1 arrest, dephosphorylation of Rb and decreased expression of cyclins D1 and D3. Increased expression of ECRG4 in aged mouse brain is observed compared with young mouse brain. The increased ECRG4 level was accompanied by elevated senescence-associated acidic beta-galactosidase activity, suggesting that ECRG4 is involved in cellular differentiation and senescence. |
Bio References |
Su T, Liu H, and Lu S. Cloning and identification of cDNA fragments related to human esophageal cancer. Zhonghua Zhong Liu Za Zhi 1998; 20:254-7.Yue CM, Deng DJ, Bi MX, et al. Expression of ECRG4, a novel esophageal cancer-related gene, downregulated by CpG island hypermethylation in human esophageal squamous cell carcinoma. World J. Gastroenterol. 2003; 9:1174-8.Kujuro Y, Suzuki N, and Kondo T. Esophageal cancer-related gene 4 is a secreted inducer of cell senescence expressed by aged CNS precursor cells. Proc. Natl. Acad. Sci. USA 2010; 107:8259-64. |
Clonality |
Polyclonal |
Gene ID |
84417 |
Gene Name |
|
Reactivity |
H, M, Rat |
Legend image 1 |
Western blot analysis of ECRG4 in HeLa cell lysate with ECRG4 antibody at (A) 1 and (B) 2 µg/mL. |
Type image 1 |
WB |
Legend image 2 |
Immunocytochemistry of ECRG4 in HeLa cells with ECRG4 antibody at 5 µg/mL. |
Type image 2 |
ICC |
Other Accession |
Q9H1Z8; 84417 |
Isotype |
IgG |
Calculated MW |
17183 |
Antigen Source |
Homo sapiens |
Target/Specificity |
ECRG4 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human ECRG4.
The immunogen is located within the last 50 amino acids of ECRG4. |
Purification |
ECRG4 Antibody is affinity chromatography purified via peptide column. |
Reconstitution & Storage |
ECRG4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Antibody Form |
Liquid |
Application Notes |
ECRG4 antibody can be used for detection of ECRG4 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ g/mL. |
Positive Control |
HeLa Cell Lysate; |
Buffer |
ECRG4 Antibody is supplied in PBS containing 0.02% sodium azide. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.