THEM4 Antibody

Q5T1C6

THEM4 Antibody: THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.

Polyclonal

THEM4

Western blot analysis of THEM4 in human liver tissue lysate with THEM4 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of THEM4 in human liver tissue with THEM4 antibody at 2.5 µg/mL.

Immunofluorescence of THEM4 in human liver tissue with THEM4 antibody at 20 µg/mL.

NP_444283; 76159293

27130

THEM4; The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.

THEM4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

THEM4 antibody can be used for detection of THEM4 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.

THEM4 Antibody is supplied in PBS containing 0.02% sodium azide.