Item no. |
ABC-ASC10730 |
Manufacturer |
Abcepta
|
Amount |
100 ug |
Category |
|
Type |
Antibody Primary |
Applications |
WB, IF, ICC, ELISA |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Available |
|
Primary Accession |
P57740 |
Application |
WB, IF, ICC, E |
Bio Background |
NUP107 Antibody: The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-107 is part of the hetero-oligomeric complex that also contains NUP160, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP107 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP107 may be an important drug target in HIV treatment. Multiple isoforms of NUP107 are known to exist. |
Bio References |
Tran EJ and Wente SR. Dynamic nuclear pore complex: life on the edge. Cell2006; 125:1041-53.Boehmer T, Enninga J, Dales S, et al. Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex. Proc. Natl. Acad. Sci. USA2003; 100:981-5. Orjalo AV, Arnaoutov A, Shen Z, et al. The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. Mol. Bio. Cell2006; 17:3806-18.Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6. |
Clonality |
Polyclonal |
Gene ID |
57122 |
Gene Name |
NUP107 |
Reactivity |
H, Rat |
Legend image 1 |
Western blot analysis of NUP107 in A549 cell lysate with NUP107 antibody at (A) 1 and (B) 2 µg/mL. |
Type image 1 |
WB |
Legend image 2 |
Immunocytochemistry of NUP107 in A549 cells with NUP107 antibody at 2.5 µg/mL. |
Type image 2 |
ICC |
Legend image 3 |
Immunofluorescence of NUP107 in A549 cells with NUP107 antibody at 10 µg/mL. |
Type image 3 |
IF |
Other Accession |
P57740; 12230339 |
Isotype |
IgG |
Calculated MW |
106374 |
Antigen Source |
Homo sapiens |
Target/Specificity |
NUP107; |
Purification |
NUP107 Antibody is affinity chromatography purified via peptide column. |
Reconstitution & Storage |
NUP107 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Antibody Form |
Liquid |
Application Notes |
NUP107 antibody can be used for detection of NUP107 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 µg/mL. For immunofluorescence start at 10 µg/mL. |
Positive Control |
A549 Cell Lysate; |
Buffer |
NUP107 Antibody is supplied in PBS containing 0.02% sodium azide. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.