Nhe-1 Antibody

P19634

Nhe-1 Antibody: The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension.

Polyclonal

SLC9A1

Western blot analysis of Nhe-1 in rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemical staining of human brain tissue using Nhe-1 antibody at 2.5 µg/mL.

Immunofluorescence of Nhe-1 in Human Brain tissue with Nhe-1 antibody at 20 µg/mL.

P19634; 127809

90763

SLC9A1; At least three isoforms of Nhe-1 are known to exist.

Nhe-1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.

Nhe-1 Antibody is supplied in PBS containing 0.02% sodium azide.