Item no. |
ABC-ASC10412 |
Manufacturer |
Abcepta
|
Amount |
100 ug |
Category |
|
Type |
Antibody Primary |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
GAPDH, GAPD, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HEL-S-162eP, OK/SW-cl.12, GAPDH Antibody: G3PD, CDABP0047 |
Available |
|
Primary Accession |
P04406 |
Application |
WB, IHC-P, IF, E |
Bio Background |
GAPDH Antibody: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots. |
Bio References |
Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90. |
Clonality |
Polyclonal |
Gene ID |
2597 |
Gene Name |
GAPDH {ECO:0000303|PubMed:2987855, ECO:0000312|HGNC:HGNC:4141} |
Reactivity |
H, M, Rat |
Legend image 1 |
Western blot analysis of GAPDH in HeLa cell lysate with GAPDH antibody at (A) 0.125, (B) 0.25 and (C) 0.5 µg/mL. |
Type image 1 |
WB |
Legend image 2 |
Immunohistochemistry of GAPDH in human liver tissue with GAPDH antibody at 10 µg/mL. |
Type image 2 |
IHC |
Legend image 3 |
Immunofluorescence of GAPDH in HeLa cells with GAPDH antibody at 10 µg/mL. |
Type image 3 |
IF |
Other Accession |
P04406; 120649 |
Isotype |
IgG |
Calculated MW |
36053 |
Antigen Source |
Homo sapiens |
Target/Specificity |
GAPDH; |
Purification |
GAPDH Antibody is affinity chromatography purified via peptide column. |
Reconstitution & Storage |
GAPDH antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Antibody Form |
Liquid |
Application Notes |
GAPDH antibody can be used for the detection of GAPDH by Western blot at 0. 125 - 0.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 10 µg/mL. For immunofluorescence start at 10 µg/mL. |
Positive Control |
HeLa Cell Lysate; |
Buffer |
GAPDH Antibody is supplied in PBS containing 0.02% sodium azide. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.