Caspase-4 Antibody

P49662

Caspase-4 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β and IL-18. Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells. More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease.

Polyclonal

CASP4 {ECO:0000303|PubMed:15123740, ECO:0000312|HGNC:HGNC:1505}

Western blot analysis of caspase-4 in human spleen cells with caspase-4 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemical staining of human spleen tissue using caspase-4 antibody at 2 µg/mL.

Immunofluorescence of Caspase-4 in A20 cells with Caspase-4 antibody at 10 µg/mL.

AAA86890; 886050

43262

CASP4; Depending on cell lines or tissues used, other cleavage products may be observed.

Caspase-4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Casp-4 antibody can be used for the detection of Caspase-4 by Western blot at 1 and 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 µg/mL. For immunofluorescence start at 10 µg/mL.

Caspase-4 Antibody is supplied in PBS containing 0.02% sodium azide.