Item no. |
ABC-ASC10191 |
Manufacturer |
Abcepta
|
Amount |
100 ug |
Category |
|
Type |
Antibody Primary |
Applications |
WB, IF, ICC, ELISA |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
RNF85, TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, MGC:3310, TRAF6 Antibody: RNF85 |
Available |
|
Primary Accession |
Q9Y4K3 |
Application |
WB, IF, ICC, E |
Bio Background |
TRAF6 Antibody: Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-κB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK. |
Bio References |
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.Wu H. Assembly of post-receptor signaling complexes for the tumor necrosis factor superfamily. Adv. Protein Chem. 2004; 68:225-79.Wajant H and Scheurich P. Analogies between Drosophila and mammalian TRAF pathways. Prog. Mol. Subcell. Biol. 2004; 34:47-72.Kobayashi T, Walsh PT, Walsh MC, et al. TRAF6 is a critical factor for dendritic cell maturation and development. Immunity 2003; 19:353-63. |
Clonality |
Polyclonal |
Gene ID |
7189 |
Gene Name |
TRAF6 |
Reactivity |
H, M, Rat |
Legend image 1 |
Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 µg/mL in the presence (A) or absence (B) of 1 µg blocking peptide. |
Type image 1 |
WB |
Legend image 2 |
Immunocytochemistry of TRAF6 in K562 cells with TRAF6 antibody at 0.5 µg/mL. |
Type image 2 |
ICC |
Legend image 3 |
Immunofluorescence of TRAF6 in K562 cells with TRAF6 antibody at 20 µg/mL. |
Type image 3 |
IF |
Other Accession |
NP_004611; 4759254 |
Isotype |
IgG |
Calculated MW |
59573 |
Antigen Source |
Homo sapiens |
Target/Specificity |
TRAF6; |
Purification |
TRAF6 Antibody is Ion exchange chromatography purified. |
Reconstitution & Storage |
TRAF6 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Antibody Form |
Liquid |
Application Notes |
TRAF6 antibody can be used for detection of TRAF6 by Western blot at 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 10 µg/mL. For immunofluorescence start at 20 µg/mL. |
Positive Control |
PC-3 Cell Lysate; K562 Cell Lysate |
Buffer |
TRAF6 Antibody is supplied in PBS containing 0.02% sodium azide. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.