Item no. |
ABC-AP9868C-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, FC, IF, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
LCAT, Lecithin-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase, Phospholipid-cholesterol acyltransferase |
Available |
|
Primary Accession |
P04180 |
Antigen Type |
Synthetic Peptide |
Application |
FC, IF, IHC-P, WB |
Bio Background |
LCAT encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. |
Bio References |
Weissglas-Volkov, D., et al. Circ Cardiovasc Genet 3(1):31-38(2010)McGeachie, M., et al. Circulation 120(24):2448-2454(2009)Chen, S.N., et al. BMC Med. Genet. 10, 111 (2009) :Voora, D., et al. Circ Cardiovasc Genet 1(2):100-106(2008) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB23365 |
Gene ID |
3931 |
Gene Name |
LCAT |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Confocal immunofluorescent analysis of LCAT Antibody (Center) (Cat. #AP9868c) with 293 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
Type image 1 |
IF |
Dilution image 1 |
1:10~50 |
Legend image 2 |
Western blot analysis of LCAT Antibody (Center) (Cat. #AP9868c) in K562 cell line lysates (35ug/lane). LCAT (arrow) was detected using the purified Pab. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
LCAT Antibody (Center) (Cat. #AP9868c) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LCAT Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:10~50 |
Legend image 4 |
LCAT Antibody (Center) (Cat. #AP9868c) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 4 |
FC |
Dilution image 4 |
1:10~50 |
Isotype |
Rabbit Ig |
Calculated MW |
49578 |
Antigen Region |
285-313 |
Antigen Source |
HUMAN |
Target/Specificity |
This LCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-313 amino acids from the Central region of human LCAT. |
NCBI Accession |
NP_000220.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.