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COL17A1 Antibody (Center)

COL17A1 Antibody (Center)

Item no.	ABC-AP9099c-ev
Manufacturer	Abcepta
Amount	80 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	COL17A1, BP180, BPAG2, LAD-1, Collagen alpha-1(XVII) chain, LABD97, 180 kDa bullous pemphigoid antigen 2, Bullous pemphigoid antigen 2, Linear IgA disease antigen 1, 120 kDa linear IgA disease antigen, 120 kDa linear IgA dermatosis antigen, 97 kDa linear IgA disease antigen, 97 kDa linear IgA bullous dermatosis antigen, 97 kDa LAD antigen, 97-LAD, Linear IgA bullous disease antigen of 97 kDa
Available
Primary Accession	Q9UMD9
Antigen Type	Synthetic Peptide
Application	WB, FC
Bio Background	COL17A1 encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane.
Bio References	Di Zenzo, G., et.al., J. Invest. Dermatol. 130 (4), 1040-1047 (2010) Ujiie, H., et.al., J. Immunol. 184 (4), 2166-2174 (2010)
Clonality	Polyclonal
Clone/Animal Names	RB22700
Gene ID	1308
Gene Name	COL17A1
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	All lanes : Anti-COL17A1 Antibody (Center) at 1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: A375 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 150 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	Overlay histogram showing A431 cells stained with AP9099c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP9099c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 2	FC
Dilution image 2	1:25
Isotype	Rabbit Ig
Calculated MW	150419
Antigen Region	475-504
Antigen Source	HUMAN
Target/Specificity	This COL17A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 475-504 amino acids from the Central region of human COL17A1.
NCBI Accession	NP_000485.3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 80 ul

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Delivery expected until 10/4/2024

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