Item no. |
ABC-AP8621b-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, FC, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
MPZ, MPP, Myelin peripheral protein, Myelin protein zero, Myelin protein P0 |
Available |
|
Primary Accession |
P25189 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IHC-P, FC |
Bio Background |
MPZ is a major structural protein of peripheral myelin. Creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae. |
Bio References |
Tachi, N., et.al., Brain Dev. 6 (6), 560-565 (1984)Hayasaka, K., et.al., Biochem. Biophys. Res. Commun. 180 (2), 515-518 (1991)Hayasaka, K., et.al., Genomics 17 (3), 755-758 (1993) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB21459 |
Gene ID |
4359 |
Gene Name |
MPZ |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-MPZ Antibody (C-term) at 1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: K562 whole cell lysateLane 3: U-87MG whole cell lysateLane 4: HL-60 whole cell lysateLane 5: CCRF-CEM whole cell lysateLane 6: Human brain whole tissue lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution. Predicted band size : 28 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:2000 |
Legend image 2 |
MPZ Antibody (C-term) (Cat. #AP8621b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MPZ Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:50~100 |
Legend image 3 |
MPZ Antibody (C-term) (Cat. #AP8621b) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 3 |
FC |
Dilution image 3 |
1:10~50 |
Isotype |
Rabbit Ig |
Calculated MW |
27555 |
Antigen Region |
186-215 |
Antigen Source |
HUMAN |
Target/Specificity |
This MPZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the C-terminal region of human MPZ. |
NCBI Accession |
NP_000521.2 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.