Item no. |
ABC-AP8181c-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
TRPM8, LTRPC6, TRPP8, Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, Transient receptor potential p8, Trp-p8, LTrpC-6, LTrpC6 |
Available |
|
Primary Accession |
Q7Z2W7 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IHC-P |
Bio Background |
TRPM8 is a receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. It is activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH and involved in menthol sensation. It is permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. |
Bio References |
Tsavaler L., Cancer Res. 61:3760-3769(2001).Kiessling A., Prostate 56:270-279(2003).Bodding, M., Cell Calcium 42 (6), 618-628 (2007) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB14417 |
Gene ID |
79054 |
Gene Name |
TRPM8 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of lysate from human brain tissue lysate, using TRPM8 Antibody (Center R536)(Cat. #AP8181c). AP8181c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Western blot analysis of TRPM8 (arrow) using rabbit polyclonal TRPM8 Antibody (Center R536)(Cat.#AP8181c).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRPM8 gene (Lane 2) (Origene Technologies). |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TRPM8 antibody (Center R536) (Cat.#AP8181c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:10~50 |
Isotype |
Rabbit Ig |
Calculated MW |
127685 |
Antigen Region |
521-552 |
Antigen Source |
HUMAN |
Target/Specificity |
This TRPM8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-552 amino acids from the Central region of human TRPM8. |
NCBI Accession |
NP_076985.4 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.