Item no. |
ABC-AP8076b-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
Serine/threonine-protein kinase Nek4, Never in mitosis A-related kinase 4, Serine/threonine-protein kinase 2, NEK4, STK2, Serine/threonine-protein kinase NRK2, NimA-related protein kinase 4 |
Available |
|
Primary Accession |
P51957 |
Antigen Type |
Synthetic Peptide |
Application |
IHC-P, WB |
Bio Background |
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway. |
Bio References |
Levedakou, E.N., et al., Oncogene 9(7):1977-1988 (1994).Lu, K.P., et al., Prog Cell Cycle Res 1, 187-205 (1995). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB2963 |
Gene ID |
6787 |
Gene Name |
NEK4 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-hNEK4-E808 at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: 293 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:2000 |
Legend image 2 |
All lanes : Anti-hNEK4-E808 at 1:2000 dilutionLane 1: Y79 whole cell lysateLane 2: 293 whole cell lysateLane 3: A549 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 95 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 2 |
WB |
Dilution image 2 |
1:2000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:50~100 |
Other Accession |
NP_003148 |
Isotype |
Rabbit Ig |
Calculated MW |
94597 |
Antigen Region |
793-824 |
Antigen Source |
HUMAN |
Target/Specificity |
This NEK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 793-824 amino acids from the C-terminal region of human NEK4. |
NCBI Accession |
NP_001180462.1; NP_003148.2 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.