Item no. |
ABC-AP7960D |
Manufacturer |
Abcepta
|
Amount |
400 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB |
Specific against |
other |
Host |
Rabbit |
Isotype |
Ig |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
MAP2K1, MEK1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1, PRKMK1, MKK1, MAP kinase kinase 1, MAPKK 1, MEK 1 |
Available |
|
Primary Accession |
Q02750 |
Antigen Type |
Synthetic Peptide |
Application |
WB |
Bio Background |
MAP2K1 is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. |
Bio References |
Zheng, B., et al., Blood 102(3):1019-1027 (2003).Li, S.P., et al., Cancer Res. 63(13):3473-3477 (2003).Zhu, X., et al., J. Neurochem. 86(1):136-142 (2003).Fringer, J., et al., J. Biol. Chem. 278(23):20612-20617 (2003).Witowsky, J.A., et al., J. Biol. Chem. 278(3):1403-1406 (2003). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB11147 |
Gene ID |
5604 |
Gene Name |
|
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-MAP2K1 Antibody at 1:1000 dilutionLane 1: HepG2 whole cell lysateLane 2: MCF-7 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Western blot analysis of MAP2K1 (arrow) using rabbit polyclonal MAP2K1 Antibody (S217) (Cat.#AP7960d).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K1 gene (Lane 2) (Origene Technologies). |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Other Accession |
P36506; Q63932; P36507; Q90891; Q05116; Q01986; P29678; P31938; Q63980; Q24324 |
Predicted |
D, Ha, M, Rb, Rat, X, C |
Isotype |
Rabbit Ig |
Calculated MW |
43439 |
Antigen Region |
196-225 |
Antigen Source |
HUMAN |
Target/Specificity |
This MEK1(MAP2K1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-225 amino acids from human MEK1(MAP2K1). |
NCBI Accession |
NP_002746.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.