Item no. |
ABC-AP7660B |
Manufacturer |
Abcepta
|
Amount |
400 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, IHC-P |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
DDR1, CD167a, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, Epithelial discoidin domain-containing receptor 1, CD167 antigen-like family member A, Cell adhesion kinase, Discoidin receptor tyrosine kinase, HGK2, Mammary carcinoma kinase 10, Protein-tyrosine kinase 3A, Epithelial discoidin domain receptor 1, TRK E, Tyrosine kinase DDR, MCK-10, Tyrosine-protein kinase CAK, Protein-tyrosine kinase RTK-6 |
Available |
|
Primary Accession |
Q08345 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IHC-P |
Bio Background |
Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. MCK10 is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. The gene is located on chromosome 6p21.3 in proximity to several HLA class I genes. |
Bio References |
Vogel, W., et al., Mol. Cell 1(1):13-23 (1997).Playford, M.P., et al., Genome Res. 6(7):620-627 (1996).Perez, J.L., et al., Oncogene 12(7):1469-1477 (1996).Valent, A., et al., Hum. Genet. 98(1):12-15 (1996).Edelhoff, S., et al., Genomics 25(1):309-311 (1995). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB1480 |
Gene ID |
780 |
Gene Name |
DDR1 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
Western blot analysis of anti-MCK10 Pab (Cat. #AP7660a) in mouse lung lysate. MCK10 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Western blot analysis of DDR1 (arrow) using MCK10 Antibody (C-term) (Cat.#AP7660b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDR1 gene (Lane 2) (Origene Technologies). |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:50~100 |
Isotype |
Rabbit Ig |
Calculated MW |
101128 |
Antigen Region |
873-904 |
Antigen Source |
HUMAN |
Target/Specificity |
This DDR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 873-904 amino acids from the C-terminal region of human DDR1. |
NCBI Accession |
NP_001189450.1; NP_001189451.1; NP_001189452.1; NP_001945.3; NP_054699.2; NP_054700.2 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.