Item no. |
ABC-AP7343c-ev |
Manufacturer |
Abcepta
|
Amount |
80 ul |
Category |
|
Type |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, FC |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
MYO1A, MIHC, BBMI, MYHL, Myosin I heavy chain, Brush border myosin I, Unconventional myosin-Ia, BBM-I |
Available |
|
Primary Accession |
Q9UBC5 |
Antigen Type |
Synthetic Peptide |
Application |
FC, WB |
Bio Background |
MYO1A belongs to the myosin superfamily. Myosins are molecular motors that, upon interaction with actin filaments, utilize energy from ATP hydrolysis to generate mechanical force. Each myosin has a conserved N-terminal motor domain that contains both ATP-binding and actin-binding sequences. Following the motor domain is a light-chain-binding 'neck' region containing 1-6 copies of a repeat element, the IQ motif, that serves as a binding site for calmodulin or other members of the EF-hand superfamily of calcium-binding proteins. At the C-terminus, each myosin class has a distinct tail domain that serves in dimerization, membrane binding, protein binding, and/or enzymatic activities and targets each myosin to its particular subcellular location. The kidney epithelial cell line, LLC-PK1-CL4 (CL4), forms a well ordered brush border (BB) on its apical surface. Experiments indicate that the brush border population of the protein turns over rapidly, while its head and tail domains interact transiently with the core actin and plasma membrane, respectively. A rapidly exchanging pool of the protein envelops an actin core bundle that, by comparison, is static in structure. |
Bio References |
D'Adamo, P., Pinna, M. Hum. Genet. 112 (3), 319-320 (2003)Hoshimaru, M., Fujio, Y. J. Biochem. 106 (3), 455-459 (1989)Durrbach, A., Collins, K. Proc. Natl. Acad. Sci. U.S.A. 93 (14), 7053-7058 (1996) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB19125 |
Gene ID |
4640 |
Gene Name |
MYO1A |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of MYO1A antibody (Center S291) (Cat.#AP7343c) in Jurkat cell line lysates (35ug/lane). MYO1A (arrow) was detected using the purified Pab. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
MYO1A Antibody (Center S291) (Cat. #AP7343c) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 2 |
FC |
Dilution image 2 |
1:10~50 |
Isotype |
Rabbit Ig |
Calculated MW |
118401 |
Antigen Region |
276-304 |
Antigen Source |
HUMAN |
Target/Specificity |
This MYO1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human MYO1A. |
NCBI Accession |
NP_001242970.1; NP_005370.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.